BIOLOGICAL EVALUATIONS OF PROTOPORPHYRIN IX, PHEOPHORBIDE a, AND ITS 1­  HYDROXYETHYL DERIVATIVESS FOR APPLICATION IN PHOTODYNAMIC THERAPY

Djalil, Asmiyenti Djaliasrin and Nurulita, Nunuk Aries and Limantara, Leenawaty and Tjahjono, Daryono Hadi (2012) BIOLOGICAL EVALUATIONS OF PROTOPORPHYRIN IX, PHEOPHORBIDE a, AND ITS 1­  HYDROXYETHYL DERIVATIVESS FOR APPLICATION IN PHOTODYNAMIC THERAPY. PEER REVIEW.

	Text (Korespondensi) Biological Evaluations of Protoporphyrin IX, Pheophorbide a, and Its 1- Hydroxyethyl Derivative for Application in Photodynamic Therapy.pdf Download (3MB)
	Text (Peer Review) Biological Evaluations of Protoporphyrin IX, Pheophorbide a, and Its 1- Hydroxyethyl Derivative For Application In Photodynamic Therapy.pdf Download (589kB)
	Text (Similiarity) BIOLOGICAL_EVALUATIONS_OF_PROTOPORPHYRIN_IX__PHEOP.pdf Download (1MB)

Abstract

Protoporphyrin  IX  (1),  pheophorbide  a  (3),  and  its  1‐hydroxyethyl  derivativess  (2,4) were studied in  vitro  as  photosensitizer  candidates  for  photodynamic therapy. Protoporphyrin IX has been indicated to have  a  cytotoxic  effect  in  the  absence  of  light  excitation.  The  dark  toxicity  of  1­4  was evaluated against normal cells (Vero), human epithelial cervix  carcinomas  (HeLa)  and  human  breast  cancer  (T47D)  cell  lines,  while  the  phototoxicity  of  1­4 was evaluated against HeLa and T47D cell lines. Moreover, the  MTT  assay  was  employed  to  evaluate  cell  viability.  The  1‐ hydroxyethyl  derivativess  showed  a  lower  dark  toxicity  in  the  three  types  of  cells  compared  to the  parent  molecules.  It  was  also  observed  that  the  parent molecules were more phototoxic than those of its 1‐hydroxyethyl derivativess.

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