Free Radical Scavenging Activity of Juice and Ethanol Extracts of Garcinia Mangostana L. Leaves

Diniatik, Diniatik and Elza, Sundhani and Nervita, Nervita (2018) Free Radical Scavenging Activity of Juice and Ethanol Extracts of Garcinia Mangostana L. Leaves. PROCEEDINGS AMCA 2018.

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Abstract

The present research was conducted to assess the free radical scavenging activity of Garcinia mangostana L leaves by DPPH (1,1, diphenyl-2 picryl hydrazyl) method. Free radical scavenging activity of juice and ethanolic extract of G. mangostana leaves were determined by using spectrophotometry UV-Vis method. Juice and 96% ethanol’s extract was concentrated by using rotary evaporator to get thick extract produces rendement about 2.571 % and 4.842 %. IC 50 values for juice and ethanol extract was 19.372 g/ml and 1.965 g/ml, it means at that concentration inhibited 50 % of free radical DPPH scavenge. IC 50 of d-α-tocopherol was 0.737 g/ml. The result showed that juice are amounted ten times less than ethanol extract. When compared with d-αtocopherol as positive control, free radical scavenging activity of juice and ethanolic extract were twenty six and three times less than d-α-tocopherol. Based on the result of anova test with significant degree 95% can be concluded that free radical scavenging activity between juice and ethanol extract were different with d-α-tocopherol.

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