Phytochemicals content, FTIR fingerprint and bioactivity of crude extract and fractions of Mesua ferrea L. leaves

Dwi, Hartanti and Zainur, Rahman Hakim and Anna, Nurlativah and Arinda, Nur Cahyani and Fadhilah, Zahra Nadia and Asmiyenti, Djaliasrin Djalil (2019) Phytochemicals content, FTIR fingerprint and bioactivity of crude extract and fractions of Mesua ferrea L. leaves. Research Journal of Life Science.

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Abstract

Mesua ferrea L. (Ceylon ironwood, Calophyllaceae) is a popular medicinal plant with a long history of use in South- and Southeast Asian folk medicines. In this study, the qualitative phytochemical analysis, FTIR fingerprint, as well as antimicrobial and antioxidant activities of the crude extract of M. ferrea leaves and its fractions were reported. The dried leaves of M. ferrea were extracted with ethanol by the re-maceration method. The crude extract was further partitioned in ethanol-water and ethyl acetate to obtain ethanol and ethyl acetate fractions, respectively. The identification of compounds in the extract and fractions was conducted according to the standard phytochemical screening method. Fourier-transform infrared (FTIR) spectroscopy was utilized to record the metabolites fingerprint of the extract and its fractions. The antimicrobial activity was evaluated with the disk diffusion method against Escherichia coli, Bacillus subtilis, Candida albicans, and Saccharomyces cerevisiae. The antioxidant activity assay was determined with 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. The crude extract of M. ferrea leaves and its fractions contained saponins, phenolic compounds, flavonoids, and terpenoids. The FTIR data supported the presence of terpenoids and glycosides in those samples. The extract and fractions demonstrated considerable antimicrobial activity against all tested bacteria and fungi, with MIC values were 3.9-31.3 μg/ml. The ethyl acetate fraction of M. ferrea leaves showed the best antioxidant activity with an IC 50 value of 49.19 μg/ml.

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