Hesperidin as a preventive resistance agent in MCF-7 breast cancer cells line resistance to doxorubicin

Rifki, Febriansah and Dyaningtyas, Dewi Pamungkas Putri and Sarmoko, Sarmoko and Nunuk, Aries Nurulita and Edy, Meiyanto and Agung, Endro Nugroho (2019) Hesperidin as a preventive resistance agent in MCF-7 breast cancer cells line resistance to doxorubicin. PEER REVIEW.

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Abstract

Objective: To evaluate of hesperidin to overcome resistance of doxorubicin in MCF-7 resistant doxorubicin cells (MCF-7/Dox) in cytotoxicity apoptosis and P-glycoprotein (Pgp) expression in combination with doxorubicin. Methods: The cytotoxic properties, 50 % inhibition concentration (IC ) and its combination with doxorubicin in MCF-7 cell lines resistant to doxorubicin (MCF-7/Dox) cells were determined using MTT assay. Apoptosis induction was examined by double staining assay using ethidium bromide-acridine orange. Immunocytochemistry assay was performed to determine the level and localization of Pgp. Results: Single treatment of hesperidin showed cytotoxic activity on MCF-7/Dox cells with IC 50 50 value of 11 µ mol/L. Thus, combination treatment from hesperidin and doxorubicin showed addictive and antagonist effect (CI>1.0). Hesperidin did not increase the apoptotic induction, but decreased the Pgp expressions level when combined with doxorubicin in low concentration. Conclusions: Hesperidin has cytotoxic effect on MCF-7/Dox cells with IC L. Hesperidin did not increased the apoptotic induction combined with doxorubicin. Cochemotherapy application of doxorubicin and hesperidin on MCF-7/Dox cells showed synergism effect through inhibition of Pgp expression.

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