DETERMINATION AND VALIDATION METHOD OF TOTAL FLAVONOID CONTENT AND TOTAL PHENOLIC CONTENT OF ETHANOLIC EXTRACT OF MANGOSTEEN (Garcinia mangostana L.) LEAVES AS NATURAL PRESERVATIVES CANDIDATE BY USING SPECTROPHOTOMETRIC METHOD

Diniatik, Diniatik and Indri, Hapsari and Milani, Tiara and Ayu, Meidyawati and Suhri, Nurhidayat (2013) DETERMINATION AND VALIDATION METHOD OF TOTAL FLAVONOID CONTENT AND TOTAL PHENOLIC CONTENT OF ETHANOLIC EXTRACT OF MANGOSTEEN (Garcinia mangostana L.) LEAVES AS NATURAL PRESERVATIVES CANDIDATE BY USING SPECTROPHOTOMETRIC METHOD. PROSIDING NETS 2013.

	Text (Cover, Editorial Team, Daftar Isi) Cover, Editorial Team, Daftar Isi.pdf Download (271kB)
	Text (Full Text) FULL TEXT PROCEEDINGS NETS 2013.pdf Download (374kB)
	Text (Peer Review) Peer Review Determination and Validation Method of Total Flavonoid Content.pdf Download (2MB)
	Text (Similiarity) Similarity-Determination and Validation Method of Total Flavonoid Content and Total Phenolic Content.pdf Download (3MB)

Abstract

Mangosteen has great potential as a natural preservative, it is necessary to ensure the content of compounds that have the potential levels. This study aimed to determine levels of total flavonoid content and total phenolic content and the validation methods. Determination and validation method used spectrophotometric methods, the methods of hydrolysis on total flavonoid content and the folin ciocalteu reagent method in the determination of phenolic total. Spectrophotometric method can be used for the determination of total flavonoid content with the results of the validation are eligible. The result of determination of total flavonoid content in 50% ethanol extract was 26.80 ± 15.9%. Spectrophotometric method can be used for the determination of total phenolic content with the results of the validation are eligible. The result of determination of total phenolic content in 50% ethanol extract was 71.94 ± 12.37%.

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