The Effect of Ethanol Extract from Binahong Leaves (Anredera Cordifolia (Ten.) Steen) on Histopathology Changes in Female Wistar Rats

Silma, Kaaffah and Fitri, Kurniwati and Anjar, Mahardian Kusuma and Asmiyenti, Djaliasrin Djalil (2019) The Effect of Ethanol Extract from Binahong Leaves (Anredera Cordifolia (Ten.) Steen) on Histopathology Changes in Female Wistar Rats. ARTIKEL PROSIDING.

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Abstract

The aim of this research is to study of the effect of ethanol extract of binahong leaves (Anredera cordifolia (Ten.) Steen) on histopathology changes in female wistar rats. Animal tests were selected using a complete randomized block design. The method to determine the dose of the extracts was according to the OECD guideline 420. Doses used in the sighting study were 300 mg/kg and 2000 mg/kg, in this test there is no death in animal test trials. Animal test in main study divided into 2 groups. Each group consisted of 5 animals tested, which is the treatment group was given a dose of 2000 mg/kg and the control group was given distilled water per oral. Each of the liver tissues and kidneys tissues were processed by paraffin block-embedded and hematoxylin eosin staining method. There were biochemical test (ALT. AST. urea) and organ weights relative to support histopathology data. The results analysis Independent T test show that ethanol extract of binahong leaves did not affect the value of histopathology (liver and kidneys). Histopathology showed the presence of fat degeneration in liver tissues on all of the treatment group and control group, whereas the results histopathological in kidneys tissues on all of the treatment group and control group were normal. The conclusion is ethanol extract of binahong leaves (Anredera cordifolia (Ten.)Steen) between dosage 300 mg/kg to 2000 mg/kg no significance toxicity effect on the liver and kidneys of rat (wistar).

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